Histone H4 Antibody (S05-8H9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20040

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20040-100ul
NBP3-20040-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-8H9

Concentration

0.3 mg/ml

View all Histone H4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Histone H4 (Uniprot # P62805)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H4 Antibody (S05-8H9)

Histone H4 Antibody (S05-8H9)

Immunohistochemistry-Paraffin: Histone H4 Antibody (S05-8H9) [NBP3-20040] -

Immunohistochemistry-Paraffin: Histone H4 Antibody (S05-8H9) [NBP3-20040] - Immunohistochemistry of paraffin-embedded Human colon cancer tissue using NBP3-20040 at dilution 1/100

Applications for Histone H4 Antibody (S05-8H9)

Application
Recommended Usage

Chromatin Immunoprecipitation

1:20-1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100-1:200

Immunoprecipitation

1:20-1:50

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 Antibody (S05-8H9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H4 Antibody (S05-8H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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