Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mammal

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.89 mg/ml

View all Histone H4 Antibodies »

Product Specifications

Immunogen

Synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4 [Swiss Prot# P62805].

Reactivity Notes

Predicted to react with most mammal species.

Modification

p Ser1

Localization

Nucleus.

Marker

H4pS1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [p Ser1] Antibody - BSA Free

Western Blot: Histone H4 [p Ser1] AntibodyBSA Free [NB21-2000]

Western Blot: Histone H4 [p Ser1] AntibodyBSA Free [NB21-2000]

Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody - BSA Free [NB21-2000]

Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody - BSA Free [NB21-2000]

Immunocytochemistry/Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in nonmitotic and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red)
Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
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Applications for Histone H4 [p Ser1] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2-5 ug per million cells

Dot Blot

0.1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000
Application Notes
This H4pS1 antibody is useful for ICC/IF, ChIP, Dot blot, and Western blot where a band is detected ~13 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.89 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4S1p

Entrez Gene IDs

121504 (Human); 320332 (Mouse)

Gene Symbol

H4-16

UniProt

P62805 (Human), P62806 (Mouse)

Additional Histone H4 Products

Product Documents for Histone H4 [p Ser1] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H4 [p Ser1] Antibody - BSA Free

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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