Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Mammal

Cited:

Mouse, Rat, Porcine

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.78 mg/ml

View all Histone H4 Antibodies »

Product Specifications

Immunogen

Synthetic aceylated peptide surrounding Lysine 5 of human Histone H4 [Swiss Prot P62805].

Reactivity Notes

Porcine reactivity reported in scientific literature (Bidne et al).

Modification

ac Lys5

Localization

Nucleus. Chromosome

Marker

H4K5ac

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys5] Antibody - BSA Free

Western Blot: Histone H4 [ac Lys5] AntibodyBSA Free [NB21-2024]

Western Blot: Histone H4 [ac Lys5] AntibodyBSA Free [NB21-2024]

Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys5] Antibody - BSA Free [NB21-2024]

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys5] Antibody - BSA Free [NB21-2024]

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
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Applications for Histone H4 [ac Lys5] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2-5 ug per million cells

Dot Blot

1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
This Histone H4 K5ac antibody is useful for ChIP, Dot blot, ICC/IF and Western blot where a band is seen ~13kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB21-2024 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.78 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K5ac

Entrez Gene IDs

121504 (Human)

Gene Symbol

H4-16

UniProt

P62805 (Human)

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys5] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H4 [ac Lys5] Antibody - BSA Free

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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