Histone H4 [ac Lys16] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB21-2077
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Mammal
Cited:
Mouse
Applications
Validated:
Chromatin Immunoprecipitation (Negative), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.62 mg/ml
Product Specifications
Immunogen
Synthetic acetylated peptide surrounding Lysine 16 of human Histone H4 [Swiss Prot P62805].
Reactivity Notes
Predicted to react with most mammal species.
Modification
ac Lys16
Localization
Nucleus. Chromosome.
Marker
H4k16ac
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [ac Lys16] Antibody - BSA Free
Western Blot: Histone H4 [ac Lys16] AntibodyBSA Free [NB21-2077]
Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys16] Antibody - BSA Free [NB21-2077]
Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Western Blot: Histone H4 [ac Lys16] AntibodyBSA Free [NB21-2077]
Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1) HeLa histone prep lysate and 2) NIH 3T3 histone prep lysate.Applications for Histone H4 [ac Lys16] Antibody - BSA Free
Application
Recommended Usage
Dot Blot
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
This H4K16ac antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot, a band is seen ~13kDa in HeLa histone prep and NIH 3T3 histone prep. In ICC/IF, nuclear staining was observed in HeLa cells. This antibody is not applicable for Chromatin Immunoprecipitation.
Reviewed Applications
Read 1 review rated 5 using NB21-2077 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.62 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K16ac
Gene Symbol
H4-16
UniProt
Additional Histone H4 Products
Product Documents for Histone H4 [ac Lys16] Antibody - BSA Free
Product Specific Notices for Histone H4 [ac Lys16] Antibody - BSA Free
EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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