Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Human, Virus

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ND1

Format

BSA Free

Concentration

1.0 mg/ml

View all HIV-1 Gag p24 Antibodies »

Product Specifications

Immunogen

HIV-1 Gag p24 C-terminal peptide.

Specificity

The antibody reacts with HIV-1 Gag p24). Specificity was verified by Western Blotting in reducing conditions with Sanofi Pasteur NEW LAV-BLOT I kit. Epitope was reported to be located within the amino acids 51-110.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HIV-1 Gag p24 Antibody (ND1) - BSA Free

Immunocytochemistry/ Immunofluorescence: HIV-1 Gag p24 Antibody (ND1) - BSA Free [NB500-473]

Immunocytochemistry/ Immunofluorescence: HIV-1 Gag p24 Antibody (ND1) - BSA Free [NB500-473]

Immunocytochemistry/Immunofluorescence: HIV-1 Gag p24 Antibody (ND1) - BSA Free [NB500-473] - Transfected p24 (green) was detected in Huh-7 cells. Cells were fixed with PFA 4%. Antibody concentration: 1:50. Dilution buffer: 1X PBS, 1% bovine serum albumin (BSA), 0.3% Triton X-100, and 0.01% sodium azide. DAPI was used for counterstaining (blue). Image submitted by a verified customer review.

Applications for HIV-1 Gag p24 Antibody (ND1) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
Purity is > 95% (by SDS-PAGE). Validated for ICC/IF from a verified customer review.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HIV-1 Gag p24

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Gene Symbol

gag

Additional HIV-1 Gag p24 Products

Product Documents for HIV-1 Gag p24 Antibody (ND1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HIV-1 Gag p24 Antibody (ND1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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