HLA DMA Antibody (6B12NB) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44302

Novus Biologicals, part of Bio-Techne
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NBP2-44302
NBP2-44302SS
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Azide Free
NBP2-44304
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6B12NB

Format

BSA Free

Concentration

1.0 mg/ml

View all HLA DMA Antibodies »

Product Specifications

Immunogen

Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]

Localization

Membranes of Late Endosomes / Lysosomes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HLA DMA Antibody (6B12NB) - BSA Free

Western Blot: HLA DMA Antibody (6B12NB)BSA Free [NBP2-44302]

Western Blot: HLA DMA Antibody (6B12NB)BSA Free [NBP2-44302]

Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)
Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (6B12NB) - BSA Free [NBP2-44302]

Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (6B12NB) - BSA Free [NBP2-44302]

Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (6B12NB) [NBP2-44302] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (6B12NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) - BSA Free [NBP2-44302]

Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) - BSA Free [NBP2-44302]

Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) [NBP2-44302] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 6B12NB). The antibody generated a punctate staining in the cytoplasm/perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.
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Applications for HLA DMA Antibody (6B12NB) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

2.5 ug / million cells

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DMA

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Entrez Gene IDs

3108 (Human)

Gene Symbol

HLA-DMA

UniProt

P28067 (Human)

Additional HLA DMA Products

Product Documents for HLA DMA Antibody (6B12NB) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HLA DMA Antibody (6B12NB) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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