HLA DOA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91993

Novus Biologicals, part of Bio-Techne
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NBP1-91993
NBP1-91993-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA DOA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DOA Antibody

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993]

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993]

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Analysis in human tonsil and liver tissues using NBP1-91993 antibody. Corresponding HLA-DOA RNA-seq data are presented for the same tissues.
Western Blot: HLA DOA Antibody [NBP1-91993]

Western Blot: HLA DOA Antibody [NBP1-91993]

Western Blot: HLA DOA Antibody [NBP1-91993] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DOA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993]

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993]

Immunohistochemistry-Paraffin: HLA DOA Antibody [NBP1-91993] - Staining of human liver shows no positivity in hepatocytes as expected.
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Applications for HLA DOA Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DOA

HLA DOA, also known as HLA class II histocompatibility antigen DO alpha chain, is a 250 amino acid protein that is 28 kDa, found in lysosomes in B cells and controls HLA-DM-mediated peptide loading on MHC class II molecules. Current research is being done on several diseases and disorders including vogt-koyanagi-harada disease, alopecia universalis, alopecia, systemic lupus erythematosus, graft versus host disease, lupus erythematosus, rheumatoid arthritis, toxoplasmosis, autoimmune thyroiditis, leishmaniasis, myocarditis, diabetes mellitus, arthritis influenza, immunodeficiency, asthma, tuberculosis, thyroiditis, interferon, and hepatitis b. Interactions with the HLA DOA protein have shown to involve ENSG00000204252 and HLA-DMA proteins in immune response antigen presentation by MHC class II, G-protein signaling N-RAS regulation pathway, immune response IL-22 signaling pathway, immune response NFAT in immune response, immune response ICOS pathway in T-helper cell, phagosome, cell adhesion molecules (CAMs), antigen processing and presentation, intestinal immune network for IgA production,and type I diabetes mellitus pathway.

Alternate Names

HLA class II histocompatibility antigen, DO alpha chain, HLA-D0-alpha, HLA-DNAHLADZ, HLA-DZA, lymphocyte antigen, major histocompatibility complex, class II, DN alpha, major histocompatibility complex, class II, DO alpha, MHC class II antigen DOA, MHC DN-alpha, MHC DZ alpha

Gene Symbol

HLA-DOA

Additional HLA DOA Products

Product Documents for HLA DOA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DOA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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