HLA DQ Antibody (K274.3G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11305

Novus Biologicals, part of Bio-Techne
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NBP3-11305-0.025mg
NBP3-11305-0.1mg
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Key Product Details

Species Reactivity

Validated:

Porcine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # K274.3G8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HLA DQ Antibodies »

Product Specifications

Immunogen

Porcine peripheral blood lymphocytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA DQ Antibody (K274.3G8)

Flow Cytometry: HLA DQ Antibody (K274.3G8) [NBP3-11305]

Flow Cytometry: HLA DQ Antibody (K274.3G8) [NBP3-11305]

Flow Cytometry: HLA DQ Antibody (K274.3G8) [NBP3-11305] - Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC.

Applications for HLA DQ Antibody (K274.3G8)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:100
Application Notes
Flow Cytometry: Use 10 uL of the suggested working dilution to label 1x10^6 cells in 100 uL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DQ

The HLADQ1 monoclonal antibody recognizes human HLA-DQ also known as MHC class II DQ monomorphic antigen. The major histocompatability complex is composed of two heterodimeric glycoproteins ( alpha and beta chains) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic, with the alpha chain showing an extremely high degree of polymorphism. HLA-DQ is expressed on B cells in the peripheral blood, weakly on activated T cells and some monocytes. HLA-DQ is absent on hematopoietic progenitors, resting T cells, erythrocytes and platelets. HLA-DQ is expressed after HLA-DR and HLA-DP in hematopoietic development. HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ T lymphocytes. Specific alleles of HLA-DQ have been linked to the pathogenesis of several autoimmune diseases (including diabetes), both as a susceptibility and resistance factor depending on the particular polymorphism. The HLADQ1 antibody has been reported to be useful for flow cytometric detection of human HLA-DQ and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).

Alternate Names

CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha

Gene Symbol

HLA-DQA1

Additional HLA DQ Products

Product Documents for HLA DQ Antibody (K274.3G8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HLA DQ Antibody (K274.3G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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