HLA DR/DP Antibody (Bra-14) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48006
![Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) - Azide and BSA Free [NBP2-48006]](https://resources.bio-techne.com/images/products/HLA-DR-DP-Antibody-Bra-14-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-48006-img0001.jpg "Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) - Azide and BSA Free [NBP2-48006]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # Bra-14
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human REH cells
Localization
Cell surface
Specificity
Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44985).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for HLA DR/DP Antibody (Bra-14) - Azide and BSA Free
Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) - Azide and BSA Free [NBP2-48006]
Immunohistochemistry-Paraffin: HLA DR/DP Antibody (Bra-14) - Azide and BSA Free [NBP2-48006] - Human Tonsil stained with HLA-DP/DR Monoclonal Antibody (Bra-14).Applications for HLA DR/DP Antibody (Bra-14) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: HLA DR/DP
Alternate Names
FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW
Gene Symbol
HLA-DRA
Additional HLA DR/DP Products
Product Documents for HLA DR/DP Antibody (Bra-14) - Azide and BSA Free
Product Specific Notices for HLA DR/DP Antibody (Bra-14) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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