HLA DRA Antibody (169-1B5.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47722
![Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722]](https://resources.bio-techne.com/images/products/HLA-DRA-Antibody-169-1B5-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47722-img0003.jpg "Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Bovine, Feline
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 169-1B5.2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human peripheral blood leukocytes (Uniprot: P01903)
Localization
Cell surface
Specificity
This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45043).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for HLA DRA Antibody (169-1B5.2) - Azide and BSA Free
Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722]
Flow Cytometry: HLA DRA Antibody (169-1B5.2) - Azide and BSA Free [NBP2-47722] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for HLA DRA Antibody (169-1B5.2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: HLA DRA
Alternate Names
FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW
Gene Symbol
HLA-DRA
Additional HLA DRA Products
Product Documents for HLA DRA Antibody (169-1B5.2) - Azide and BSA Free
Product Specific Notices for HLA DRA Antibody (169-1B5.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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