HLA DRA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33458

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-33458
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA DRA Antibodies »

Product Summary for HLA DRA Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA DRA. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%), Canine (87%).

Localization

Membrane |

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA DRA Antibody

Immunocytochemistry/ Immunofluorescence: HLA DRA Antibody [NBP1-33458]

Immunocytochemistry/ Immunofluorescence: HLA DRA Antibody [NBP1-33458]

Immunocytochemistry/Immunofluorescence: HLA DRA Antibody [NBP1-33458] - Raji cells were fixed in 100% Methenol for 5 min. Green- HLA-DRA protein stained by HLA-DRA antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar- 10 um.
Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458]

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for HLA DRA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DRA

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Alternate Names

FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW

Gene Symbol

HLA-DRA

Additional HLA DRA Products

Product Documents for HLA DRA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA DRA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.