HLA DRB1 Antibody (HLA-DRB/7795R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20479
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # HLA-DRB/7795R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human HLA DRB1 protein
Localization
Cell surface.
Specificity
This monoclonal antibody reacts with a 28kDa chain of HLA DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Human lymphoid tissue.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for HLA DRB1 Antibody (HLA-DRB/7795R)
Western blot analysis of HDLM-2 cell lysate using HLA DRB1 antibody (HLA-DRB/7795R).
HLA DRB1 Antibody (HLA-DRB/7795R)
Formalin-fixed, paraffin-embedded human lymph node stained with HLA DRB1 antibody (HLA-DRB/7795R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
HLA DRB1 Antibody (HLA-DRB/7795R)
Formalin-fixed, paraffin-embedded human tonsil stained with HLA DRB1 antibody (HLA-DRB/7795R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for HLA DRB1 Antibody (HLA-DRB/7795R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA-DRB1
Long Name
Major histocompatibility complex, class II, DR beta 1
Alternate Names
DRB1, HLA-DR1B, HLA-DRB, SS1
Gene Symbol
HLA-DRB1
Additional HLA-DRB1 Products
Product Documents for HLA DRB1 Antibody (HLA-DRB/7795R)
Product Specific Notices for HLA DRB1 Antibody (HLA-DRB/7795R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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