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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rHLA-DRB/7198

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human HLA DRB1 protein

Localization

Cell surface.

Specificity

This monoclonal antibody reacts with a 28kDa chain of HLA DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: Raji, Ramos, Daudi or HuT78 cells. Human lymphoid tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

HLA DRB1 Antibody (rHLA-DRB/7198)

Formalin-fixed, paraffin-embedded human tonsil stained with HLA DRB1 antibody (rHLA-DRB/7198). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HLA-DRB1

HLA DRB1 can be used for HLA typing.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Gene Symbol

HLA-DRB1

Additional HLA-DRB1 Products

Product Documents for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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