HLA DRB1 Antibody (SPM289) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34756

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM289

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all HLA DRB1 Antibodies »

Product Summary for HLA DRB1 Antibody (SPM289) - Azide and BSA Free

Immunogen

Activated human peripheral blood mononuclear cells

Localization

Cell surface

Specificity

This monoclonal antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45311).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HLA DRB1 Antibody (SPM289) - Azide and BSA Free

Western Blot: HLA DRB1 Antibody (SPM289)Azide and BSA Free [NBP2-34756]

Western Blot: HLA DRB1 Antibody (SPM289)Azide and BSA Free [NBP2-34756]

Western Blot: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM289).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Formalin-paraffin human tonsil stained with HLA-DR MAb (SPM289).
Flow Cytometry: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756]

Flow Cytometry: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756]

Flow Cytometry: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM289).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for HLA DRB1 Antibody (SPM289) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HLA DRB1

HLA DRB1 can be used for HLA typing.

Alternate Names

Clone P2-beta-3, DR1, DR-1, DR12, DR-12, DR13, DR-13, DR14, DR-14, DR16, DR-16, DR4, DR-4, DR5, DR-5, DR7, DR-7, DR8, DR-8, DR9, DR-9, DRB1, DRw10, DRw11, DRw8, DW2.2/DR2.2, FLJ75017, FLJ76359, HLA class II antigen beta chain, HLA class II histocompatibility antigen, DR-1 beta chain, HLA-DR1B, HLA-DRB, HLA-DRB1*, HLA-DRB2, HLA-DR-beta 1, human leucocyte antigen DRB1, leucocyte antigen DR beta 1 chain, leucocyte antigen DRB1, lymphocyte antigen DRB1, major histocompatibility complex, class II, DR beta 1, MHC class II antigen DRB1*1, MHC class II antigen DRB1*10, MHC class II antigen DRB1*11, MHC class II antigen DRB1*12, MHC class II antigen DRB1*13, MHC class II antigen DRB1*14, MHC class II antigen DRB1*15, MHC class II antigen DRB1*16, MHC class II antigen DRB1*3, MHC class II antigen DRB1*4, MHC class II antigen DRB1*7, MHC class II antigen DRB1*8, MHC class II antigen DRB1*9, MHC class II antigen HLA-DR13, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR-beta cell surface glycoprotein, MHC class II HLA-DRw10-beta, SS1

Gene Symbol

HLA-DRB1

Additional HLA DRB1 Products

Product Documents for HLA DRB1 Antibody (SPM289) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DRB1 Antibody (SPM289) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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