HLA DRB3 Antibody (Bra30) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08911
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Baboon, Canine, Chimpanzee, Monkey, Squirrel
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # Bra30
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for HLA DRB3 Antibody (Bra30) - Azide and BSA Free
Immunogen
Human REH cells (non-T, non-B leukemia cell line)
Localization
Cell Surface
Specificity
This monoclonal antibody reacts with the HLA-DRB3 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07168).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for HLA DRB3 Antibody (Bra30) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: HLA DRB3
Alternate Names
DR7, HLA class II histocompatibility antigen, DR beta 3 chain, HLA class II histocompatibility antigen, DRB1-7 beta chain, HLA-DR3B, HLA-DR52, HLA-DRB1, human leucocyte antigen DRB3, major histocompatibility complex, class II, DR beta 3, MGC117330, MHC class II antigen DR beta 3 chain, MHC class II antigen DRB3, MHC class II HLA-DR beta 3 chain
Gene Symbol
HLA-DRB3
Additional HLA DRB3 Products
Product Documents for HLA DRB3 Antibody (Bra30) - Azide and BSA Free
Product Specific Notices for HLA DRB3 Antibody (Bra30) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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