HLA DRB5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14093

Novus Biologicals, part of Bio-Techne
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NBP2-14093
NBP2-14093-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA DRB5 Antibodies »

Product Summary for HLA DRB5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVGESFTVQRRVEPKVTVYPARTQT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DRB5 Antibody

Western Blot: HLA DRB5 Antibody [NBP2-14093]

Western Blot: HLA DRB5 Antibody [NBP2-14093]

Western Blot: HLA DRB5 Antibody [NBP2-14093] - Analysis in human cell line HDLM-2.
Immunohistochemistry-Paraffin: HLA DRB5 Antibody [NBP2-14093]

Immunohistochemistry-Paraffin: HLA DRB5 Antibody [NBP2-14093]

Immunohistochemistry-Paraffin: HLA DRB5 Antibody [NBP2-14093] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Applications for HLA DRB5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DRB5

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Alternate Names

DR beta-5, DR2-beta-2, DR9, DR-9, Dw2, FLJ76359, HLA class II histocompatibility antigen, DR beta 5 chain, HLA class II histocompatibility antigen, DR-5 beta chain, HLA class II histocompatibility antigen, DRB1-9 beta chain, HLA-DRB, human leucocyte antigen DRB5, leukocyte antigen class II, major histocompatibility complex, class II, DR beta 5, MHC class II antigen DRB1*9, MHC class II antigen DRB5, MHC class II HLA beta chain, MHC HLA-DR-beta cell surface glycoprotein, MHC HLA-DR-beta chain

Gene Symbol

HLA-DRB5

Additional HLA DRB5 Products

Product Documents for HLA DRB5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DRB5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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