HLA G Antibody (4H84) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55297

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H84

Format

BSA Free

Concentration

1.0 mg/ml

View all HLA-G Antibodies »

Product Specifications

Immunogen

amino acids 61-83 of HLA G Antibody (4H84) of human origin.

Specificity

The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA G Antibody (4H84) - BSA Free

Immunohistochemistry-Paraffin: HLA G Antibody (4H84) - BSA Free [NB110-55297]

Immunohistochemistry-Paraffin: HLA G Antibody (4H84) - BSA Free [NB110-55297]

Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).
Western Blot: HLA G Antibody (4H84)BSA Free [NB110-55297]

Western Blot: HLA G Antibody (4H84)BSA Free [NB110-55297]

Western Blot: HLA G Antibody (4H84) [NB110-55297] - Analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.
Western Blot: HLA G Antibody (4H84)BSA Free [NB110-55297]

Western Blot: HLA G Antibody (4H84)BSA Free [NB110-55297]

Western Blot: HLA G Antibody (4H84) [NB110-55297] - HLA G Antibody (4h84) [NB110-55297] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody (NB110-55297) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Applications for HLA G Antibody (4H84) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
ELISA: The antibody 4H84 has been tested as the capture antibody in a sandwich ELISA for analysis of heavy chains of all HLA-G isoforms in combination with antibody MEM-G/1 (cat. no. NB500-613).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA-G

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Long Name

Major Histocompatibility Complex, Class I, G

Alternate Names

HLA G, HLA-6.0, HLAG, MHC Class I Antigen G, MHC-G

Entrez Gene IDs

3135 (Human)

Gene Symbol

HLA-G

Additional HLA-G Products

Product Documents for HLA G Antibody (4H84) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA G Antibody (4H84) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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