HLF Antibody (M2)

Novus Biologicals, part of Bio-Techne | Catalog # H00003131-M04

Novus Biologicals, part of Bio-Techne
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H00003131-M04
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLF Antibodies »

Product Specifications

Immunogen

HLF (AAH36093, 1 a.a. ~ 295 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKMSRPLPLNPTFIPPPYGVLRSLLENPLKLPLHHEDAFSKDKDKEKKLDDESNSPTVPQSAFLGPTLWDKTLPYDGDTFQLEYMDLEEFLSENGIPPSPSQHDHSPHPPGLQPASSAAPSVMDLSSRASAPLHPGIPSPNCMQSPIRPGQLLPANRNTPSPIDPDTIQVPVGYEPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPMIKKARKVFIPDDLKDDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENSALRQEVADLRKELGKCKNILAKYEARHGPL

Specificity

HLF - hepatic leukemia factor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HLF Antibody (M2)

Western Blot: HLF Antibody (M2) [H00003131-M04]

Western Blot: HLF Antibody (M2) [H00003131-M04]

Western Blot: HLF Antibody (M2) [H00003131-M04] - Western blot analysis of HLF over-expressed 293 cell line, cotransfected with HLF Validated Chimera RNAi or non-transfected control. Blot probed with H00003131-M04. GAPDH (36.1 kDa) used as loading control.
Immunocytochemistry/ Immunofluorescence: HLF Antibody (M2) [H00003131-M04]

Immunocytochemistry/ Immunofluorescence: HLF Antibody (M2) [H00003131-M04]

Immunocytochemistry/Immunofluorescence: HLF Antibody (M2) [H00003131-M04] - Immunofluorescence of to HLF ( Cat# H00003131-M04 ) on HeLa cell .
Western Blot: HLF Antibody (M2) [H00003131-M04]

Western Blot: HLF Antibody (M2) [H00003131-M04]

Western Blot: HLF Antibody (M2) [H00003131-M04] - analysis of HLF expression in transfected 293T cell line by HLF monoclonal antibody (M04), clone M2. Lane 1: HLF transfected lysate (33.2 KDa). Lane 2: Non-transfected lysate.

Applications for HLF Antibody (M2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLF

This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Alternate Names

hepatic leukemia factor, MGC33822

Entrez Gene IDs

3131 (Human)

Gene Symbol

HLF

OMIM

142385 (Human)

UniProt

AAH36093 (Human)

Additional HLF Products

Product Documents for HLF Antibody (M2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLF Antibody (M2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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