HMBS Antibody (3E8)
Novus Biologicals, part of Bio-Techne | Catalog # H00003145-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HMBS (AAH00520.1, 1 a.a. ~ 361 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKIPDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH
Specificity
HMBS (3E8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HMBS Antibody (3E8)
![Western Blot: HMBS Antibody (3E8) [H00003145-M01]](https://resources.bio-techne.com/images/products/HMBS-Antibody-3E8-Western-Blot-H00003145-M01-img0004.jpg "Western Blot: HMBS Antibody (3E8) [H00003145-M01]")
Western Blot: HMBS Antibody (3E8) [H00003145-M01]
Western Blot: HMBS Antibody (3E8) [H00003145-M01] - Analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.![Western Blot: HMBS Antibody (3E8) [H00003145-M01]](https://resources.bio-techne.com/images/products/HMBS-Antibody-3E8-Western-Blot-H00003145-M01-img0001.jpg "Western Blot: HMBS Antibody (3E8) [H00003145-M01]")
Western Blot: HMBS Antibody (3E8) [H00003145-M01]
Western Blot: HMBS Antibody (3E8) [H00003145-M01] - analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.
Sandwich ELISA: HMBS Antibody (3E8) [H00003145-M01] - Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.
Applications for HMBS Antibody (3E8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HMBS
Alternate Names
hydroxymethylbilane synthasePre-uroporphyrinogen synthase, PBGDPBG-D, PORC, porphobilinogen deaminase, porphyria, acute; Chester type, UPSEC 2.5.1.61, uroporphyrinogen I synthase, uroporphyrinogen I synthetase
Entrez Gene IDs
3145 (Human)
Gene Symbol
HMBS
OMIM
609806 (Human)
UniProt
Additional HMBS Products
Product Documents for HMBS Antibody (3E8)
Product Specific Notices for HMBS Antibody (3E8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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