Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F4

Concentration

1 mg/ml

View all HMBS Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HMBS (NP_000181) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMBS Antibody (OTI3F4)

Western Blot: HMBS Antibody (OTI3F4) [NBP2-45976]

Western Blot: HMBS Antibody (OTI3F4) [NBP2-45976]

Western Blot: HMBS Antibody (3F4) [NBP2-45976] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS.
Immunohistochemistry: HMBS Antibody (OTI3F4) [NBP2-45976]

Immunohistochemistry: HMBS Antibody (OTI3F4) [NBP2-45976]

Immunohistochemistry: HMBS Antibody (3F4) [NBP2-45976] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: HMBS Antibody (OTI3F4) [NBP2-45976]

Immunohistochemistry: HMBS Antibody (OTI3F4) [NBP2-45976]

Immunohistochemistry: HMBS Antibody (3F4) [NBP2-45976] - Analysis of Adenocarcinoma of Human ovary tissue.
View 3 more images

Applications for HMBS Antibody (OTI3F4)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HMBS

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

hydroxymethylbilane synthasePre-uroporphyrinogen synthase, PBGDPBG-D, PORC, porphobilinogen deaminase, porphyria, acute; Chester type, UPSEC 2.5.1.61, uroporphyrinogen I synthase, uroporphyrinogen I synthetase

Gene Symbol

HMBS

Additional HMBS Products

Product Documents for HMBS Antibody (OTI3F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HMBS Antibody (OTI3F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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