HMG-CoA Reductase/HMGCR Antibody (CL0260)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61617

Novus Biologicals, part of Bio-Techne
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NBP2-61617
NBP2-61617-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0260

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMG-CoA Reductase/HMGCR Antibodies »

Product Summary for HMG-CoA Reductase/HMGCR Antibody (CL0260)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTT

Epitope

TLMETHERGVSIRRQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HMG-CoA Reductase/HMGCR Antibody (CL0260)

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Analysis in human liver and skeletal muscle tissues using NBP2-61617 antibody. Corresponding HMGCR RNA-seq data are presented for the same tissues.
Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP2-61617] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 4 more images

Applications for HMG-CoA Reductase/HMGCR Antibody (CL0260)

Application
Recommended Usage

Immunohistochemistry

1:20 -1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMG-CoA Reductase/HMGCR

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

3-Hydroxy-3-Methylglutaryl-CoA Reductase

Alternate Names

HMGCR, Hydroxymethylglutaryl-CoA Reductase, LDLCQ3

Gene Symbol

HMGCR

Additional HMG-CoA Reductase/HMGCR Products

Product Documents for HMG-CoA Reductase/HMGCR Antibody (CL0260)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMG-CoA Reductase/HMGCR Antibody (CL0260)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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