HMGB3/HMG4 Antibody (JE63-39)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32437
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE63-39
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human HMG4 aa 50-150. (Uniprot: O15347)
Localization
Nucleus, Chromosome, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HMGB3/HMG4 Antibody (JE63-39)
Western Blot: HMGB3/HMG4 Antibody (JE63-39) [NBP3-32437]
Western Blot: HMGB3/HMG4 Antibody (JE63-39) [NBP3-32437] - Western blot analysis of HMGB3/HMG4 on different lysates with Rabbit anti-HMGB3/HMG4 antibody (NBP3-32437) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for HMGB3/HMG4 Antibody (JE63-39)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HMGB3
Long Name
High Mobility Group Box 3
Alternate Names
HMG2A, HMG4
Gene Symbol
HMGB3
Additional HMGB3 Products
Product Documents for HMGB3/HMG4 Antibody (JE63-39)
Product Specific Notices for HMGB3/HMG4 Antibody (JE63-39)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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