HMGCL Antibody (4F4-D1)

Novus Biologicals, part of Bio-Techne | Catalog # H00003155-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F4-D1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMGCL Antibodies »

Product Specifications

Immunogen

HMGCL (AAH10570, 1 a.a. ~ 325 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

Specificity

HMGCL - 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HMGCL Antibody (4F4-D1)

Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01]

Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01]

Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01]

Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01]

Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - GCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).

Applications for HMGCL Antibody (4F4-D1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMGCL

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Entrez Gene IDs

3155 (Human)

Gene Symbol

HMGCL

OMIM

246450 (Human)

UniProt

AAH10570 (Human)

Additional HMGCL Products

Product Documents for HMGCL Antibody (4F4-D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGCL Antibody (4F4-D1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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