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HMGCL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58026

Novus Biologicals, part of Bio-Techne
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NBP1-58026

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to HMGCL(3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)) The peptide sequence was selected from the N terminal of HMGCL (NP_000182). Peptide sequence WVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAA The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for HMGCL Antibody

Western Blot: HMGCL Antibody [NBP1-58026]

Western Blot: HMGCL Antibody [NBP1-58026]

Western Blot: HMGCL Antibody [NBP1-58026] - analysis of HMGCL in LPS+ATP treated bone marrrow derived macrophages using anti-HMGCL antibody. Image from verified customer review.
Western Blot: HMGCL Antibody [NBP1-58026]

Western Blot: HMGCL Antibody [NBP1-58026]

Western Blot: HMGCL Antibody [NBP1-58026] - Titration: 1.25ug/ml Positive Control: Human Liver.

Applications for HMGCL Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-58026 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGCL

The deficiency of HMGCL is related to an autosomal recessive branched chain organic aciduria.

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Gene Symbol

HMGCL

UniProt

Additional HMGCL Products

Product Documents for HMGCL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGCL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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