HMGCL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83251

Novus Biologicals, part of Bio-Techne
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NBP1-83251
NBP1-83251-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMGCL Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%). Reactivity reported in scientific literature (PMID: 23082721)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGCL Antibody

Western Blot: HMGCL Antibody [NBP1-83251]

Western Blot: HMGCL Antibody [NBP1-83251]

Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining in human liver and skin tissues using anti-HMGCL antibody. Corresponding HMGCL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human liver shows high expression.
View 1 more images

Applications for HMGCL Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGCL

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Gene Symbol

HMGCL

Additional HMGCL Products

Product Documents for HMGCL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGCL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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