HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45269

Novus Biologicals, part of Bio-Techne
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NBP2-45269SS
NBP2-45269
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (98%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B11NB

Format

BSA Free

Concentration

1.0 mg/ml

View all HNF-3 alpha/FoxA1 Antibodies »

Product Specifications

Immunogen

Partial recombinant human FOXA1 protein (between amino acids 350-500) .

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB)BSA Free [NBP2-45269]

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB)BSA Free [NBP2-45269]

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human prostate.
View 4 more images

Applications for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

Application
Recommended Usage

Flow Cytometry

25 ug/ml

Immunocytochemistry/ Immunofluorescence

1:25 - 1:50

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FoxA1 (forkhead box A1), also called HNF-3a (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

UniProt

P55317

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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