HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45269
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Predicted:
Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3B11NB
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human FOXA1 protein (between amino acids 350-500) .
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB)BSA Free [NBP2-45269]
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectivelyImmunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free [NBP2-45269]
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human prostate.Applications for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
Application
Recommended Usage
Flow Cytometry
25 ug/ml
Immunocytochemistry/ Immunofluorescence
1:25 - 1:50
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2-4 ug/ml
Reviewed Applications
Read 1 review rated 4 using NBP2-45269 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HNF-3 alpha/FoxA1
Long Name
Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
Alternate Names
FoxA1, HNF3 alpha, TCF3A
Gene Symbol
FOXA1
UniProt
Additional HNF-3 alpha/FoxA1 Products
Product Documents for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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