HNF-3 alpha/FoxA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38628

Novus Biologicals, part of Bio-Techne
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NBP2-38628
NBP2-38628-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HNF-3 alpha/FoxA1 Antibodies »

Product Summary for HNF-3 alpha/FoxA1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QTLDHSGATATGGASELKTPASSTAPPISSGPGALASVPASHPAHGLAPHESQLHLKGDPHYSFNHP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Analysis in human prostate and endometrium tissues. Corresponding HNF-3 alpha/FoxA1 RNA-seq data are presented for the same tissues.
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Applications for HNF-3 alpha/FoxA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

UniProt

P55317

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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