HNF-3 beta/FoxA2 Antibody (7E6)

Novus Biologicals, part of Bio-Techne | Catalog # H00003170-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7E6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HNF-3 beta/FoxA2 Antibodies »

Product Specifications

Immunogen

FOXA2 (NP_068556, 363 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS

Specificity

FOXA2 - forkhead box A2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HNF-3 beta/FoxA2 Antibody (7E6)

Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody (M01), clone 7E6.Lane 1: FOXA2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of monoclonal antibody to FOXA2 on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01]

Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi ( Cat # H00003170-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody (M01), clone 7E6 (Cat # H00003170-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for HNF-3 beta/FoxA2 Antibody (7E6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human)

Gene Symbol

FOXA2

OMIM

600288 (Human)

UniProt

NP_068556 (Human)

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody (7E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 beta/FoxA2 Antibody (7E6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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