HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70904

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3C10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HNF-3 beta/FoxA2 Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10)Azide and BSA Free [NBP2-70904]

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10)Azide and BSA Free [NBP2-70904]

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904]

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904]

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.
Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904]

Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904]

Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody.
View 4 more images

Applications for HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

FOXA2 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Gene Symbol

FOXA2

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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