HNF-4 alpha/NR2A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1783

Novus Biologicals, part of Bio-Techne
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NB100-1783
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Primate

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all HNF-4 alpha/NR2A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence RLSKTLVDMDMADY-C corresponding to N-Terminus according to NP_849180.1, NP_000448.3, NP_849181.1.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID18561282).

Specificity

This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for HNF-4 alpha/NR2A1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.5 ug/ml
Application Notes
WB: Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1). Primary incubation was 1 hour.

Reviewed Applications

Read 1 review rated 1 using NB100-1783 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

NP_849180.1 (Human)

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HNF-4 alpha/NR2A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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