Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F12

Concentration

0.72 mg/ml

View all HNF-6/ONECUT1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens one cut homeobox 1 (ONECUT1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-6/ONECUT1 Antibody (OTI4F12)

Western Blot: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Western Blot: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Western Blot: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6.
Immunocytochemistry/ Immunofluorescence: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Immunocytochemistry/ Immunofluorescence: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Immunocytochemistry/Immunofluorescence: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6.
Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody (OTI4F12) [NBP1-47903]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody.

Applications for HNF-6/ONECUT1 Antibody (OTI4F12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-6/ONECUT1

The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.

Long Name

Hepatocyte Nuclear Factor 6

Alternate Names

HNF-6A, HNF6, OC-1, ONECUT1

Entrez Gene IDs

3175 (Human)

Gene Symbol

ONECUT1

UniProt

Q9UBC0 (Human)

Additional HNF-6/ONECUT1 Products

Product Documents for HNF-6/ONECUT1 Antibody (OTI4F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HNF-6/ONECUT1 Antibody (OTI4F12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.