hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80777

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # DP3B3

Format

Azide and BSA Free

Concentration

1 mg/ml

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Product Summary for hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

Immunogen

Bacterially expressed human hnRNP A2 fusion protein.

Reactivity Notes

Mouse, Human, Chicken, Canine, Rat, Monkey, Bovine and Hamster.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

Western Blot Shows Human hnRNP A2B1 Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and hnRNP A2B1 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with hnRNP A2B1 Antibody (Catalog # NBP2-80777) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for hnRNP A2B1 (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: hnRNP A2B1 Antibody (DP3B3)Azide and BSA Free [NBP2-80777]

Western Blot: hnRNP A2B1 Antibody (DP3B3)Azide and BSA Free [NBP2-80777]

Western Blot: hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free [NBP2-80777] - Whole cell protein from PC12 (lane 1), NIH-3T3 (lane 2), A431 (lane 3) and HeLa (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP A2B1 at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free [NBP2-80777]

Immunocytochemistry/ Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free [NBP2-80777]

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free [NBP2-80777] - The hnRNP A2B1 antibody was tested in HeLa cells at a 5ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.
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Applications for hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP A2B1

RNA polymerase II transcripts in the nucleus are in complex with several different proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins act in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA ranslation, and turnover.2. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform.3 The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weights (34-43 kDa).3 hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells; however, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin, and the supraoptic ucleus of the hypothalamus.4 Expression of hnRNP-A2/B1 proteins was found also in lung development and its over expression correlated with lung cancer.5

Alternate Names

DKFZp779B0244, FLJ22720, heterogeneous nuclear ribonucleoprotein A2/B1, heterogeneous nuclear ribonucleoprotein B1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1hnRNP A2/B1, HNRPB1, nuclear ribonucleoprotein particle A2 protein, RNPA2, SNRPB1

Gene Symbol

HNRNPA2B1

Additional hnRNP A2B1 Products

Product Documents for hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP A2B1 Antibody (DP3B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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