hnRNP K Antibody (3G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15177

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15177
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all hnRNP K Antibodies »

Product Summary for hnRNP K Antibody (3G7)

Immunogen

A synthesized peptide derived from human hnRNP K. UniProt ID (P61978).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP K Antibody (3G7)

Western Blot: hnRNP K Antibody (3G7) [NBP3-15177]

Western Blot: hnRNP K Antibody (3G7) [NBP3-15177]

Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.
Immunocytochemistry/ Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177]

Immunocytochemistry/ Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177]

Immunocytochemistry/Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177] - Immunofluorescence staining of A549 cells with at hnRNP K antibody 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177]

Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177]

Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Applications for hnRNP K Antibody (3G7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: hnRNP K

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Alternate Names

dC-stretch binding protein, FLJ41122, heterogeneous nuclear ribonucleoprotein K, hnRNP K, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNPHNRPKCSBP

Gene Symbol

HNRNPK

Additional hnRNP K Products

Product Documents for hnRNP K Antibody (3G7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP K Antibody (3G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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