Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # N10 P2D3*G2

Format

BSA Free

Concentration

1 mg/ml

View all hnRNP K Antibodies »

Product Summary for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

Immunogen

Ovalbumin-conjugated synthetic peptide SVKQYSGKFF (C-terminal sequence)

Reactivity Notes

Immunogen peptide sequence is common to mouse, rat, rabbit, chicken, sheep, dog, pig, horse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

Western Blot: hnRNP K Antibody (N10 P2D3*G2)BSA Free [NBP2-50424]

Western Blot: hnRNP K Antibody (N10 P2D3*G2)BSA Free [NBP2-50424]

Western Blot: hnRNP K Antibody (N10 P2D3G2) [NBP2-50424] - Detection of hnRNP K isoform 1, a strong band at 51 kDa is observed.
Immunohistochemistry: hnRNP K Antibody (N10 P2D3*G2) - BSA Free [NBP2-50424]

Immunohistochemistry: hnRNP K Antibody (N10 P2D3*G2) - BSA Free [NBP2-50424]

Immunohistochemistry: hnRNP K Antibody (N10 P2D3G2) [NBP2-50424] - Staining of formalin-fixed, paraffin-embedded tissue sections. Strong nuclear immunostaining was observed in normal skin (A). Nuclear and cytoplasmic immunostaining was observed in breast carcinoma (B). This is in agreement with existing literature as hnRNPK shows aberrant localisation in tumours.

Applications for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): IHC - formalin-fixed, paraffin-embedded colorectal cancer sections; WB - Jurkat cell lysate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP K

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Alternate Names

dC-stretch binding protein, FLJ41122, heterogeneous nuclear ribonucleoprotein K, hnRNP K, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNPHNRPKCSBP

Gene Symbol

HNRNPK

Additional hnRNP K Products

Product Documents for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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