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hnRNP-Q Antibody (9C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26294

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26294-100ul
NBP3-26294-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9C7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for hnRNP-Q Antibody (9C7)

Immunogen

A synthesized peptide derived from Human hnRNP-Q [UniProt O60506]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP-Q Antibody (9C7)

hnRNP-Q Antibody (9C7)

Immunohistochemistry: hnRNP-Q Antibody (9C7) [NBP3-26294] -

Immunohistochemistry: hnRNP-Q Antibody (9C7) [NBP3-26294] - Image of hnRNP-Q Antibody (9C7) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
hnRNP-Q Antibody (9C7)

Immunohistochemistry: hnRNP-Q Antibody (9C7) [NBP3-26294] -

Immunohistochemistry: hnRNP-Q Antibody (9C7) [NBP3-26294] - Image of hnRNP-Q Antibody (9C7) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
hnRNP-Q Antibody (9C7)

Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody (9C7) [NBP3-26294] -

Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody (9C7) [NBP3-26294] - Staining of Hela Cells with hnRNP-Q Antibody (9C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for hnRNP-Q Antibody (9C7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: hnRNP-Q

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins

Alternate Names

dJ3J17.2, Glycine- and tyrosine-rich RNA-binding protein, GRYRBP, GRY-RBPNS1-associated protein 1, heterogeneous nuclear ribonucleoprotein Q, hnRNP Q, hnRNP-Q, HNRPQ, HNRPQ1, NSAP1FLJ31626, PP68, synaptotagmin binding, cytoplasmic RNA interacting protein, Synaptotagmin-binding, cytoplasmic RNA-interacting protein

Gene Symbol

SYNCRIP

Additional hnRNP-Q Products

Product Documents for hnRNP-Q Antibody (9C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for hnRNP-Q Antibody (9C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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