hnRNP U Antibody (3R3F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16363

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16363-20ul
NBP3-16363-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3R3F1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all hnRNP U Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human hnRNP U (Q00839). MSSSPVNVKKLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRSGAGLEQEAAAGGDEEEEEEEEEEEGISALD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP U Antibody (3R3F1)

Western Blot: hnRNP U Antibody (3R3F1) [NBP3-16363]

Western Blot: hnRNP U Antibody (3R3F1) [NBP3-16363]

Western Blot: hnRNP U Antibody (3R3F1) [NBP3-16363] - Western blot analysis of extracts of various cell lines, using hnRNP U Rabbit mAb (NBP3-16363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: hnRNP U Antibody (3R3F1) [NBP3-16363]

Immunocytochemistry/ Immunofluorescence: hnRNP U Antibody (3R3F1) [NBP3-16363]

Immunocytochemistry/Immunofluorescence: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunofluorescence analysis of U-2 OS cells using hnRNP U Rabbit mAb (NBP3-16363) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: hnRNP U Antibody (3R3F1) [NBP3-16363]

Immunohistochemistry-Paraffin: hnRNP U Antibody (3R3F1) [NBP3-16363]

Immunohistochemistry-Paraffin: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry of paraffin-embedded mouse liver using hnRNP U Rabbit mAb (NBP3-16363) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for hnRNP U Antibody (3R3F1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP U

hnRNP U belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Alternate Names

heterogeneous nuclear ribonucleoprotein U, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), hnRNP U, HNRPU, p120, p120 nuclear protein, pp120, SAFA, SAF-AhnRNPU, Scaffold attachment factor A, U21.1

Gene Symbol

HNRNPU

Additional hnRNP U Products

Product Documents for hnRNP U Antibody (3R3F1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP U Antibody (3R3F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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