Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6B8-2F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HO-1/HMOX1/HSP32 Antibodies »

Product Summary for HO-1/HMOX1/HSP32 Antibody (6B8-2F2)

Immunogen

His-tagged Rat HO-1

Localization

Microsome , Endoplasmic Reticulum

Specificity

Detects 32kDa. Does not cross-react with HO-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HO-1/HMOX1/HSP32 Antibody (6B8-2F2)

Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338]

Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338]

Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.
Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338]

Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338]

Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody, Clone 6B8-2F2 (NBP2-59338). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody (NBP2-59338) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.

Applications for HO-1/HMOX1/HSP32 Antibody (6B8-2F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody (6B8-2F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HO-1/HMOX1/HSP32 Antibody (6B8-2F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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