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HO-1/HMOX1/HSP32 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77460

Novus Biologicals, part of Bio-Techne
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NBP1-77460
NBP1-77460SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.17 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human Heme Oxygenase 1 protein (between residues 225-275)

Localization

Microsome. Endoplasmic reticulum.

Marker

Oxidative Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HO-1/HMOX1/HSP32 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody - BSA Free [NBP1-77460]

Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody - BSA Free [NBP1-77460]

Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody - BSA Free [NBP1-77460]

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody - BSA Free [NBP1-77460]

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - Analysis of Heme Oxygenase 1 in mouse kidney using DAB with hematoxylin counterstain.

Applications for HO-1/HMOX1/HSP32 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
Based on customer feedback, this antibody may not be suitable for use in western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.17 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HO-1/HMOX1/HSP32

HO-1 (also know as Heme oxygenase-1, HMOX1, HSP32), a member of the heme oxygenase family, is an enzyme for heme catabolism and cleaves heme to form biliverdin. This inducible protein reacts from hypoxia, cytokines, and oxidative stress. HO-1 is itself an antioxidant that protects cells from ROS (reactive oxygen species) by regulating ROS levels. HO-1 has been shown to inhibit the pathogenesis of immune related inflammatory diseases as well as cardiovascular disease (CVD).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Entrez Gene IDs

3162 (Human); 15368 (Rat)

Gene Symbol

HMOX1

UniProt

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HO-1/HMOX1/HSP32 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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