HO-1/HMOX1/HSP32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89964

Novus Biologicals, part of Bio-Techne
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NBP1-89964
NBP1-89964-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HO-1/HMOX1/HSP32 Antibodies »

Product Summary for HO-1/HMOX1/HSP32 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Marker

Oxidative Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HO-1/HMOX1/HSP32 Antibody

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining in human spleen and skeletal muscle tissues using NBP1-89964 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Analysis in human cell lines A-549 and MCF-7 using anti-HMOX1 antibody. Corresponding HMOX1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human Fallopian tube shows shows no positivity in glandular cells as expected.
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Applications for HO-1/HMOX1/HSP32 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HO-1/HMOX1/HSP32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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