HOOK3 Antibody (3A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00084376-M04
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HOOK3 (NP_115786.1, 622 a.a. ~ 718 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR
Specificity
Reacts with hook homolog 3 (Drosophila).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for HOOK3 Antibody (3A5)
![Western Blot: HOOK3 Antibody (3A5) [H00084376-M04]](https://resources.bio-techne.com/images/products/HOOK3-Antibody-3A5-Western-Blot-H00084376-M04-img0003.jpg "Western Blot: HOOK3 Antibody (3A5) [H00084376-M04]")
Western Blot: HOOK3 Antibody (3A5) [H00084376-M04]
Western Blot: HOOK3 Antibody (3A5) [H00084376-M04] - Analysis of HOOK3 expression in transfected 293T cell line by HOOK3 monoclonal antibody (M04), clone 3A5. Lane 1: HOOK3 transfected lysate (Predicted MW: 83.1 KDa). Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: HOOK3 Antibody (3A5) [H00084376-M04]](https://resources.bio-techne.com/images/products/HOOK3-Antibody-3A5-Immunohistochemistry-Paraffin-H00084376-M04-img0001.jpg "Immunohistochemistry-Paraffin: HOOK3 Antibody (3A5) [H00084376-M04]")
Immunohistochemistry-Paraffin: HOOK3 Antibody (3A5) [H00084376-M04]
Immunohistochemistry-Paraffin: HOOK3 Antibody (3A5) [H00084376-M04] - Analysis of monoclonal antibody to HOOK3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml![ELISA: HOOK3 Antibody (3A5) [H00084376-M04]](https://resources.bio-techne.com/images/products/HOOK3-Antibody-3A5-Sandwich-ELISA-H00084376-M04-img0002.jpg "ELISA: HOOK3 Antibody (3A5) [H00084376-M04]")
ELISA: HOOK3 Antibody (3A5) [H00084376-M04]
ELISA: HOOK3 Antibody (3A5) [H00084376-M04] - Detection limit for recombinant GST tagged HOOK3 is 1 ng/ml as a capture antibody.Applications for HOOK3 Antibody (3A5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HOOK3
Alternate Names
FLJ31058, hHK3, HK3h-hook3, hook homolog 3 (Drosophila), protein Hook homolog 3
Entrez Gene IDs
84376 (Human)
Gene Symbol
HOOK3
UniProt
Additional HOOK3 Products
Product Documents for HOOK3 Antibody (3A5)
Product Specific Notices for HOOK3 Antibody (3A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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