Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Porcine, Bovine, Ovine

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HOOK3 Antibodies »

Product Specifications

Immunogen

The epitope recognized by this antibody maps to a region between residue 425 to 475 of human Protein Hook homolog 3 using the numbering given in entry Q86VS8.2 (GeneID 84376).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HOOK3 Antibody

Western Blot: HOOK3 Antibody [NBP2-44279]

Western Blot: HOOK3 Antibody [NBP2-44279]

Western Blot: HOOK3 Antibody [NBP2-44279] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44279 was used for IP at 6 ug per reaction. HOOK3 was also Immunoprecipitated by another rabbit anti-HOOK3 antibody.
Western Blot: HOOK3 Antibody [NBP2-44279]

Western Blot: HOOK3 Antibody [NBP2-44279]

Western Blot: HOOK3 Antibody [NBP2-44279] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer.

Applications for HOOK3 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HOOK3

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM]

Alternate Names

FLJ31058, hHK3, HK3h-hook3, hook homolog 3 (Drosophila), protein Hook homolog 3

Gene Symbol

HOOK3

Additional HOOK3 Products

Product Documents for HOOK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HOOK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.