Hormone-sensitive Lipase/HSL Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76735

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (93%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Hormone-sensitive Lipase/HSL Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe. Amino Acid Squence: FPEGFHPRRSSQGA

Specificity

Multiple isoforms of Lipe are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hormone-sensitive Lipase/HSL Antibody - BSA Free

Western Blot: Hormone-sensitive Lipase/HSL AntibodyBSA Free [NBP1-76735]

Western Blot: Hormone-sensitive Lipase/HSL AntibodyBSA Free [NBP1-76735]

Western Blot: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue lysate with HSL antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735]

Immunocytochemistry/ Immunofluorescence: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735]

Immunocytochemistry/Immunofluorescence: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735]

Immunohistochemistry-Paraffin: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735]

Immunohistochemistry-Paraffin: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue with HSL antibody at 2.5 ug/ml.

Applications for Hormone-sensitive Lipase/HSL Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hormone-sensitive Lipase/HSL

Although initially described as an adipocyte-specific triacylglycerol lipase, lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that lipe plays a major role in adipocyte proliferation.

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Gene Symbol

LIPE

UniProt

ABA03168 (Human)

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Hormone-sensitive Lipase/HSL Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hormone-sensitive Lipase/HSL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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