Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05459
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]](https://resources.bio-techne.com/images/products/Hormone-sensitive-Lipase-HSL-%5Bp-Ser660%5D-Antibody-Western-Blot-NBP3-05459-img0001.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
Immunogen
A synthetic phosphorylated peptide around S660 of human Hormone-sensitive Lipase/HSL (NP_005348.2). QTSRS
Modification
p Ser660
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]](https://resources.bio-techne.com/images/products/Hormone-sensitive-Lipase-HSL-[p-Ser660]-Antibody-Western-Blot-NBP3-05459-img0001.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]")
Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] AntibodyAzide and BSA Free [NBP3-05459]
Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.Applications for Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Hormone-sensitive Lipase/HSL
HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.
Alternate Names
Hormonesensitive Lipase, HSL, LHS, LIPE
Gene Symbol
LIPE
Additional Hormone-sensitive Lipase/HSL Products
Product Documents for Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
Product Specific Notices for Hormone-sensitive Lipase/HSL [p Ser660] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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