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HOXA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32515

Novus Biologicals, part of Bio-Techne
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NBP2-32515

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for HOXA4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NTKMRSSNSASASAGPPGKAQTQSPHLHPHPHPSTSTPVPSSI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HOXA4 Antibody

Western Blot: HOXA4 Antibody [NBP2-32515]

Western Blot: HOXA4 Antibody [NBP2-32515]

Western Blot: HOXA4 Antibody [NBP2-32515] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: HOXA4 Antibody [NBP2-32515]

Immunocytochemistry/ Immunofluorescence: HOXA4 Antibody [NBP2-32515]

Immunocytochemistry/Immunofluorescence: HOXA4 Antibody [NBP2-32515] - Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: HOXA4 Antibody [NBP2-32515]

Immunohistochemistry-Paraffin: HOXA4 Antibody [NBP2-32515]

Immunohistochemistry-Paraffin: HOXA4 Antibody [NBP2-32515] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Applications for HOXA4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOXA4

HOXA4 is 320 amino acids long, weighing approximately 46 kDa, that functions as a sequence-specific transcription factor which focuses on the specific positional identities of cells on the anterior-posterior axis, which is all part of a developmental regulatory system, specifically the embryonic nervous system. Current studies are being done on several diseases and disorders including abdominal aortic aneuryms, megacolon, myeloid leukemia, Hirschsprung's disease, hypospadias, teratocarcinoma, ovarian cancer, neuroblastoma, and pancreatitis. HOXA4 has also been shown to have interactions with NFE2.

Alternate Names

homeo box A4, homeobox A4, Homeobox protein Hox-1.4, Homeobox protein Hox-1D, homeobox protein Hox-A4, HOX1, Hox-1.4-like protein, HOX1DDfd-like protein

Gene Symbol

HOXA4

Additional HOXA4 Products

Product Documents for HOXA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HOXA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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