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HOXC6 Antibody (JE66-64)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32441

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32441

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE66-64

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within Human HOXC6 aa 1-100 / 235. (Uniprot: P09630)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOXC6 Antibody (JE66-64)

HOXC6 Antibody (JE66-64)

Western Blot: HOXC6 Antibody (JE66-64) [NBP3-32441]

Western Blot: HOXC6 Antibody (JE66-64) [NBP3-32441] - Western blot analysis of HOXC6 on different lysates with Rabbit anti-HOXC6 antibody (NBP3-32441) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: HUVEC cell lysate
Lane 4: Jurkat cell lysate
Lane 5: PMVEC cell lysate
Lane 6: Rat spinal cord tissue lysate
Lane 7: Rat bladder tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 1 minute 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32441) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for HOXC6 Antibody (JE66-64)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOXC6

The Hox proteins play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. The mammalian Hox gene complex consists of 39 genes that are located on four linkage groups, which are dispersed over four chromosomes. Hox genes that occupy the same relative position along the 5' to 3' coordinate (trans-paralogous genes) are more similar in sequence and expression pattern than adjacent Hox genes on the same chromosome. HoxC6 sequence-specific transcription factor is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HoxC6 may be a novel potential therapeutic target for prostate cancer.

Alternate Names

CP25, HHO.C8, homeo box 3C, homeo box C6, homeo box C8 protein, homeobox C6, Homeobox protein CP25, Homeobox protein HHO.C8, Homeobox protein Hox-3C, homeobox protein Hox-C6, HOX3CHOX3

Gene Symbol

HOXC6

Additional HOXC6 Products

Product Documents for HOXC6 Antibody (JE66-64)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HOXC6 Antibody (JE66-64)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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