HP1 alpha Antibody (3A11F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52434

Novus Biologicals, part of Bio-Techne
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NBP2-52434
NBP2-52434-0.025mg
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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A11F8

Format

BSA Free

Concentration

1 mg/ml

View all HP1 alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HP1 alpha (AA:1-191) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HP1 alpha Antibody (3A11F8) - BSA Free

Western Blot: HP1 alpha Antibody (3A11F8)BSA Free [NBP2-52434]

Western Blot: HP1 alpha Antibody (3A11F8)BSA Free [NBP2-52434]

Western Blot: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (3A11F8) - BSA Free [NBP2-52434]

Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (3A11F8) - BSA Free [NBP2-52434]

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: HP1 alpha Antibody (3A11F8) - BSA Free [NBP2-52434]

Flow Cytometry: HP1 alpha Antibody (3A11F8) - BSA Free [NBP2-52434]

Flow Cytometry: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Flow cytometric analysis of Hela cells using CBX5 mouse mAb (green) and negative control (red).
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Applications for HP1 alpha Antibody (3A11F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HP1 alpha

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Gene Symbol

CBX5

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody (3A11F8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HP1 alpha Antibody (3A11F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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