HP1 alpha Antibody (8C3M6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16267

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16267-100ul
NBP3-16267-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8C3M6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HP1 alpha Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 92-191 of human HP1 alpha (P45973). SNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HP1 alpha Antibody (8C3M6)

Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Western blot analysis of extracts of various cell lines, using HP1 alphaRabbit mAb (NBP3-16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Immunohistochemistry-Paraffin: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry of paraffin-embedded mouse testis using HP1 alphaRabbit mAb (NBP3-16267) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267]

Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Western blot analysis of extracts of various cell lines, using HP1 alphaRabbit mAb (NBP3-16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for HP1 alpha Antibody (8C3M6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:20-1:50

Immunoprecipitation

0.5 μg-4 μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HP1 alpha

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Gene Symbol

CBX5

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody (8C3M6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HP1 alpha Antibody (8C3M6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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