HP1 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2518

Novus Biologicals, part of Bio-Techne
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NB100-2518
NB100-2518SS
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Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HP1 alpha Antibodies »

Product Specifications

Immunogen

A synthetic peptide containing an N-terminal region of human HP1 alpha (within residues 1-100). [Swiss-Prot P45973]

Reactivity Notes

Expected to react with Mouse, Arabidopsis suecica, Rye and Triticum aestivum due to sequence homology.

Localization

Nuclear

Marker

Heterochromatin Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HP1 alpha Antibody

Western Blot: HP1 alpha Antibody [NB100-2518]

Western Blot: HP1 alpha Antibody [NB100-2518]

Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody [NB100-2518]

Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody [NB100-2518]

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow (Intracellular): HP1 alpha Antibody [NB100-2518]

Flow (Intracellular): HP1 alpha Antibody [NB100-2518]

Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with rIgG (AF488) isotype control (blue).

Applications for HP1 alpha Antibody

Application
Recommended Usage

Flow (Intracellular)

1:500

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:500 - 1:1000
Application Notes
In Western blot a band is seen at ~20 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HP1 alpha

HP1 alpha (heterochromatin protein 1 homolog alpha or CBX5) is a component of heterochromatin that recognizes and binds H3K9me (histone H3 tails methylated at 'Lys-9') for facilitating epigenetic repression and is excluded from chromatin once histone H3 is phosphorylated at Tyr 41 position (H3Y41ph). HP1 alpha can interact with LBR (lamin-B receptor) thereby mediating heterochromatin association with inner nuclear membrane and its interaction with MIS12 complex proteins is implicated in functional kinetochore formation. HP1 alpha is a highly conserved nonhistone protein with chromatin organization modifier domain or chromodomain, and is localized in the nucleus where it participates in centromeric/pericentromeric heterochromatin and associates with chromosomes during mitotic metaphase/anaphase facilitating accurate cell division. It also serve as a common gene expression signature shared by human mature oocytes and embryonic stem cells, and through interactions with ATRX as well as H3.3, HP1 alpha acts a key regulator of ES-cell telomere chromatin. HP1 alpha is up-regulated in breast cancer cells and in lung cancer, it is essential to the maintenance of aggressiveness as well as stem cell like properties of tumor cells.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Gene Symbol

CBX5

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HP1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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