Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HP1 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468).

Marker

Heterochromatin Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HP1 alpha Antibody

Western Blot: HP1 alpha Antibody [NB110-40623]

Western Blot: HP1 alpha Antibody [NB110-40623]

Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]

Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]

Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for HP1 alpha Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:200-1:10000

Immunohistochemistry-Paraffin

1:200-1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Chip, IP reactivity reported in (PMID: 23045645). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HP1 alpha

CBX5 (chromobox protein homolog 5), also known as HP1 (heterochromatin protein 1 homolog alpha), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression. Alternate names for CBX5 include HP1 alpha, antigen p25, HP1Hs-alpha, and HP1A.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Entrez Gene IDs

23468 (Human)

Gene Symbol

CBX5

UniProt

P45973 (Human)

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HP1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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