HPRT Antibody (5F11A7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37245

Novus Biologicals, part of Bio-Techne
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NBP2-37245
NBP2-37245-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F11A7

Format

BSA Free

Concentration

1 mg/ml

View all HPRT Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HPRT (AA: FULL(1-218)) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HPRT Antibody (5F11A7) - BSA Free

Western Blot: HPRT Antibody (5F11A7)BSA Free [NBP2-37245]

Western Blot: HPRT Antibody (5F11A7)BSA Free [NBP2-37245]

Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) - BSA Free [NBP2-37245]

Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) - BSA Free [NBP2-37245]

Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) [NBP2-37245] - Analysis of kidney tissues using HPRT1 mouse mAb with DAB staining.
Flow (Intracellular): HPRT Antibody (5F11A7) - BSA Free [NBP2-37245]

Flow (Intracellular): HPRT Antibody (5F11A7) - BSA Free [NBP2-37245]

Flow (Intracellular): HPRT Antibody (5F11A7) [NBP2-37245] - An intracellular stain was performed on HeLa cells with HPRT Antibody (5F11A7) NBP2-37245APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
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Applications for HPRT Antibody (5F11A7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Gene Symbol

HPRT1

Additional HPRT Products

Product Documents for HPRT Antibody (5F11A7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HPRT Antibody (5F11A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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